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spheroplast buffer  (AMS Biotechnology)


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    Structured Review

    AMS Biotechnology spheroplast buffer
    Spheroplast Buffer, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spheroplast+buffer/TAE+buffer/bio_rxiv__2025__02__14__638270-379-76-103
    Average 96 stars, based on 43 article reviews
    spheroplast buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number
    Article Snippet: .. Cells were harvested at 3993 g for 5 min and washed twice with 50 ml of H 2 O with a 2 min centrifugation at 3146 g. Cells were incubated for 10 min at 75 rpm agitation and 30°C in 2 ml/g wet weight of alkaline solution (100 mM Tris, 10 mM DTT) and pelleted as also in the following washing step at 1861 g for 5 min. After washing with 6.7 ml/g wet weight in spheroplast buffer (20 mM KH 2 PO 4 pH 7.4, 1.2 M sorbitol), cells were gently resuspended in the same buffer containing 6/6.6 mg/ml Zymolyase (R) 20T (Amsbio 120491-1) to digest cell walls for 30 min at 30°C and 75 rpm agitation. ..

    Article Title: The LKB1-like kinase Elm1 controls septin hourglass assembly and stability by regulating filament pairing
    Article Snippet: .. After centrifugation, the cell pellet was resuspended in spheroplast buffer (10 mM PIPES, 1.2 M sorbitol, pH 6.5) containing 0.3 mg/mL zymolyase-100T (Amsbio, Oxfordshire, UK) and incubated at 35°C with moderate shaking for 1 hour. ..

    Article Title: Critical roles of a RhoGEF-anillin module in septin architectural remodeling during cytokinesis
    Article Snippet: .. After centrifugation, the cell pellet was resuspended in spheroplast buffer (10 mM PIPES,1.2 M sorbitol, pH 6.5) containing 0.3 mg/mL zymolyase-100T (Amsbio, Oxfordshire, UK) and incubated at 35°C with moderate shaking for 1 h. Sephroplasts were washed three times with the spheroplast buffer and then mounted to a polylysine-coated coverslip. ..

    Incubation:

    Article Title: Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number
    Article Snippet: .. Cells were harvested at 3993 g for 5 min and washed twice with 50 ml of H 2 O with a 2 min centrifugation at 3146 g. Cells were incubated for 10 min at 75 rpm agitation and 30°C in 2 ml/g wet weight of alkaline solution (100 mM Tris, 10 mM DTT) and pelleted as also in the following washing step at 1861 g for 5 min. After washing with 6.7 ml/g wet weight in spheroplast buffer (20 mM KH 2 PO 4 pH 7.4, 1.2 M sorbitol), cells were gently resuspended in the same buffer containing 6/6.6 mg/ml Zymolyase (R) 20T (Amsbio 120491-1) to digest cell walls for 30 min at 30°C and 75 rpm agitation. ..

    Article Title: The LKB1-like kinase Elm1 controls septin hourglass assembly and stability by regulating filament pairing
    Article Snippet: .. After centrifugation, the cell pellet was resuspended in spheroplast buffer (10 mM PIPES, 1.2 M sorbitol, pH 6.5) containing 0.3 mg/mL zymolyase-100T (Amsbio, Oxfordshire, UK) and incubated at 35°C with moderate shaking for 1 hour. ..

    Article Title: Critical roles of a RhoGEF-anillin module in septin architectural remodeling during cytokinesis
    Article Snippet: .. After centrifugation, the cell pellet was resuspended in spheroplast buffer (10 mM PIPES,1.2 M sorbitol, pH 6.5) containing 0.3 mg/mL zymolyase-100T (Amsbio, Oxfordshire, UK) and incubated at 35°C with moderate shaking for 1 h. Sephroplasts were washed three times with the spheroplast buffer and then mounted to a polylysine-coated coverslip. ..



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